Genolyze in The Fifth Baltic Bone and Cartilage Conference in Naantali, Finland, 1-3 September, 2005
MICROARRAYS IN PHARMATEST BONE ASSAY
Together Pharmatest Services Ltd and Genolyze Ltd can provide a unique product combination of Human Bone Cell in vitro bone assay and DNA microarray expression data. Pharmatest offers assays, e.g., in oncology, osteoporosis and osteoarthritis. Genolyze provides a complete analysis of the microarray data by applying valid statistical, computational and visualization methods tailored for the assay, and also to meet the MIAME standards in MAGE compatible form.
The innovative concept of the Pharmatest experiment setup includes the use of human osteoclast cells in vitro. In the assay, bone resorption is measured as a result of a drug treatment. Gene expression microarrays, done with the same cultures, identify those genes whose expression the Pharmatest assay compounds affect. The study reveals how the cells respond to the compounds and at which doses. A possible response can be detectable at the gene level before it is visible or even measurable in the culture. Consequently, gene level information of the bone cells could give an extra dimension when making decisions on, e.g., what compounds should be selected for further development.
MICROARRAYS IN BONE RESEARCH
Gene expression and early microarray studies have shown that there are at least 200 genes directly or indirectly involved in bone metabolism which may contribute to either development or prevention of osteoporosis [1]. Recently, the first in vivo microarray study, directly measuring osteoporosis in humans, was reported by Liu et al. [2]. Together these results have shown microarray techniques to be a very promising approach to study bone disease-related genes and the mechanism of their action.
A recent bone study using microarrays, describes the gene expression in human osteoclast differentiation process. This study is presented here in this conference [3].
In microarrays, large amounts of data are generated. To process all the data, computational and statistical expertise is essential. Genolyze Ltd has specialized in applying advanced statistical and computational methods and offers a complete solution for customers planning a microarray experiment: design of an assay, complete data analysis and aided result interpretation.
Genolyze offers fast and reliable service helping to increase the efficiency of studies with massive and complex data files within Life Sciences. Genolyze has recently extended the applications expertise into working with data sets from all High Throughput experiments in genomics, proteomics and other -omic studies within the Systems Biology field.
1. Ho NC et al. (2000) A skeletal gene database. J Bone Miner Res 15 (11), 2095-2122
2. Liu Y-Z et al. (2005) A novel pathophysiological mechanism for osteoporosis suggested by an in vivo gene expression study of circulating monocytes. The J Biol Chem, Vol. 280 (32), 29011-29016
3. Vuorikoski HJ et al. (2005) Identification of the genes differentially expressed in human osteoclasts, dendritic cells, endothelial cells and macrophages from a common precursor by microarray analysis.
Poster presentation in ECTS - IBMS 2nd Joint Meeting, Geneva, Switzerland, 25-29 June, 2005
Poster presentation in The Fifth Baltic Bone and Cartilage Conference in Naantali, Finland, 1-3 September, 2005, Poster "Vuorikoski"
|